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72755

5-(Bromomethyl)fluorescein

Name: 5-(Bromomethyl)fluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)

Synonyme(s) :

5-BMF

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About This Item

Formule empirique (notation de Hill) :

C₂₁H₁₃BrO₅

Numéro CAS:

148942-72-7

Poids moléculaire :

425.23

Code UNSPSC :

12352200

ID de substance PubChem :

329764330

Gamme de produits

BioReagent

Essai

≥95% (sum of tautomers, GC)

Fluorescence

λ_ex 492 nm; λ_em 515 nm in 0.1 M Tris pH 9.0

Adéquation

suitable for fluorescence

Chaîne SMILES

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(CBr)ccc45)c1

InChI

1S/C21H13BrO5/c22-10-11-1-4-15-14(7-11)20(25)27-21(15)16-5-2-12(23)8-18(16)26-19-9-13(24)3-6-17(19)21/h1-9,23-24H,10H2

Clé InChI

OQHKPJAZGYJYTB-UHFFFAOYSA-N

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Autres remarques

Labeling of nucleosides and nucleotides at the N-atom; labeling of carboxylic acids: shows very strong absorption and fluorescence.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number

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Labeling the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum at Glu-439 with 5-(bromomethyl)fluorescein.

H I Stefanova et al.

Biochemistry, 32(23), 6095-6103 (1993-06-15)

The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoichiometry of one label per ATPase molecule was found, which was unaffected by the presence of ATP. Labeling resulted in a 60% decrease in ATPase activity. Sequencing identified

Effect of magnetic nanoparticles on tobacco BY-2 cell suspension culture.

Olga Krystofova et al.

International journal of environmental research and public health, 10(1), 47-71 (2013-01-25)

Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silver nanoparticles), electronics (high-resolution imaging, logical circuits

Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.

U Alexiev et al.

Proceedings of the National Academy of Sciences of the United States of America, 92(2), 372-376 (1995-01-17)

The pH-indicator dye fluorescein was covalently bound to the surface of the purple membrane at position 72 on the extracellular side of bacteriorhopsin and at positions 101, 105, 160, or 231 on the cytoplasmic side by reacting bromomethylfluorescein with the

Localization of the hinge region of the Ca(2+)-ATPase of sarcoplasmic reticulum using resonance energy transfer.

K J Baker et al.

Biochimica et biophysica acta, 1192(1), 53-60 (1994-06-01)

The Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum can be labelled at Cys-670 and Cys-674 with 5-[[2-[(iodoacetyl) amino]ethyl]amino]naphthalene-1-sulphonic acid (IAEDANS). Resonance energy transfer has been used to measure the distance between Cys-670/Cys-674 and Glu-439 labelled with 5-(bromomethyl)fluorescein as 40 A. The

5-Bromomethyl fluorescein (5-BMF) for derivatization of carboxyl containing analytes for use with laser-induced fluorescence detection.

P S Mukherjee et al.

Pharmaceutical research, 12(6), 930-936 (1995-06-01)

5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. The reagent possessed high molar absorptivity and quantum yield and it's excitation maximum matched the intense 488.0 nm emission

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