66540049

RCi002-A

Human iPS Cell Line

• Synonyme(s) : EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
• Code UNSPSC : 41106509

Propriétés

• Source biologique: human erythroblast
• Méthode de reprogrammation: Sendai virus
• Description: age (N/A)
• Fabricant/nom de marque: EBiSC^™
• Sexe: female
• Mode de croissance: adherent (pluripotent)
• Technique(s): cell culture | stem cell: suitable
• Maladie(s) pertinente(s): erythromelalgia
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origine de la lignée cellulaire

Depositor
Pfizer Limited - Pfizer

Description de la lignée cellulaire

Derivation
Primary cell type: Erythroblast
Primary cell developmental stage: Adult
Passage number reprogrammed: NA
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi002-B

Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)POU5F1 (OCT-4)(+) TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: 46XX
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Liaison

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Procédure de repiquage

Medium: mTeSR^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37°C

Informations légales

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable