349M-9

ZAP-70 (2F3.2) Mouse Monoclonal Antibody

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: 2F3.2, monoclonal
• Description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• Forme: buffered aqueous solution
• Espèces réactives: human
• Conditionnement: vial of 0.1 mL concentrate (349M-94); vial of 0.5 mL concentrate (349M-95); bottle of 1.0 mL predilute (349M-97); vial of 1.0 mL concentrate (349M-96); bottle of 7.0 mL predilute (349M-98)
• Fabricant/nom de marque: Cell Marque^®
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• Isotype: IgG2a
• Contrôle: chronic lymphocytic leukemia
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Visualisation: cytoplasmic
• Informations sur le gène: human ... ZAP70(7535)

Description

Description générale

Zeta-associated protein-70 (ZAP-70), a member of the Syk family of tyrosine kinases, has an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development.[1] ZAP-70 protein is not expressed in most normal mature B-cells, but is expressed in various B- and T-cell lymphomas.[1] Attempts to implement ZAP-70 analysis in clinical flow cytometry laboratories have been fraught with difficulty; this approach is technically challenging and results often have been unreliable. Detection of ZAP-70 protein by immunohistochemistry using tissue sections of chronic lymphocytic leukemia/small lymphocyctic lymphoma (CLL/SLL) is an easy and inexpensive technique.[1] The clinical course of CLL/SLL is extremely variable: in some patients it is aggressive and requires chemotherapy within a relatively short time of diagnosis; in others it is indolent and asymptomatic and may not require treatment for many years[2]. It has recently been demonstrated that the mutational status of the immunoglobulin heavy-chain variable-region (IgVH) genes identifies 2 distinct groups of CLL/SLL: the patients with unmutated IgVH have an unfavorable clinical course, whereas those with mutated IgVH experience a favorable course. The reasons for the differences in the clinical course of CLL patients with mutated and unmutated IgVH genes are not clear. ZAP-70 expression is the best discriminator of mutated and unmutated IgVH genes[3], ZAP-70 positive (>20% tumor cells) being Ig-unmutated and ZAP-70 negative being Ig-mutated. Wiestner et al.[3] demonstrated that ZAP-70 expression levels assessed by means of Western blotting, real-time RT-PCR and immunohistochemistry are closely correlated with IgVH mutational status.

Qualité

IVD

IVD

IVD

NA

Liaison

ZAP-70 Positive Control Slides, Product No. 349S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Forme physique

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Notes préparatoires

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Autres remarques

For Technical Service please contact: 800-665-7284 or email: [email protected]

Informations légales

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable