01673

Acriflavine

BioReagent, suitable for fluorescence, ≥90% (AT)

• Synonyme(s) : 3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-diaminoacridine (proflavine), Euflavine, Trypaflavine Neutral
• Formule empirique (notation de Hill) : C₁₄H₁₄ClN₃
• Numéro CAS: 8048-52-0
• Poids moléculaire : 259.73
• UNSPSC Code: 12171500
• PubChem Substance ID: 329747342
• NACRES: NA.32
• MDL number: MFCD00064307

Propriétés

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90% (AT)
• form: powder
• composition: Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC
• solubility: H₂O: 0.33 g/mL (lit.)(lit.)
• fluorescence: λ_ex 463 nm; λ_em 490 nm in ethanol
• suitability: suitable for fluorescence
• SMILES string: [Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46
• InChI: 1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H
• InChI key: PEJLNXHANOHNSU-UHFFFAOYSA-N

Description

Application

Acriflavine is used to fluorescently label high molecular weight RNA molecules .
Excitation and emission wavelengths in various solvents :

• Methanol: λex = 424 nm; λem = 518 nm
• Ethanol: λex = 426 nm; λem = 524 nm
• Propanol: λex = 430 nm; λem = 512 nm
• Butanol: λex = 430 nm; λem = 526 nm
• Formamide: λex = 434 nm; λem = 524 nm
• Glycerol: λex = 432 nm; λem = 540 nm
• Water: λex = 416 nm; λem = 514 nm

Insoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .

Other Notes

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously[1]

Informations sur la sécurité

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H302,H315,H319,H335,H400
• pcodes: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves