PPB011

Solution saline tamponnée au Tris

powder, pH 7.6, pHast Pack^™

• Synonyme(s) : TBS
• Numéro MDL: MFCD00132476
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• product name: Solution saline tamponnée au Tris, pH 7.6, pHast Pack^™, powder
• Gamme de produits: pHast Pack^™
• Niveau de qualité: 100
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 7.6
• Solubilité: soluble, clear, colorless
• Traces de cations: Fe: ~10 ppm
• Activité étrangère: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Température de stockage: room temp

Description

Description générale

Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline can be used as a wash buffer for:

• Enzyme Linked Immunosorbent assay (ELISA)
• Western Blot and other protein applications
• Suitable for use in cell culture (recommend to sterile filter)
• Immunohistochemistry
• Sample preparation
• Resuspension and sonication of cells

Caractéristiques et avantages

• Ready for use, just dissolve in ultrapure water
• No calculations, measuring, and pH adjustments needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1