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95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Synonyme(s) :

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Formule empirique (notation de Hill):
C37H27N3Na2O9S3
Numéro CAS:
28983-56-4
Poids moléculaire :
799.80
Numéro C.I. (Colour Index):
42780
Numéro CE :
249-352-9
Numéro MDL:
MFCD00058509
Code UNSPSC :
12171500
ID de substance PubChem :
329770433
Nomenclature NACRES :
MA.02

Qualité

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Niveau de qualité

200

Forme

powder or crystals

pH

9.4-14

Solubilité

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

Clé InChI

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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Description générale

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

>572.0 °F - Pensky-Martens closed cup

Point d'éclair (°C)

> 300 °C - Pensky-Martens closed cup

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCK8203
BCCB9353
BCBV2236
BCBR0621V
BCBM0641V

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Consulter la Bibliothèque de documents

Juan Guo et al.
Journal of hazardous materials, 225-226, 63-73 (2012-05-23)
Fe nanoparticles@graphene composites (FGC) are successfully synthesized by using graphene oxide (GO) as a supporting matrix. GO is first treated with Fe(3+) to form Fe(3+)@GO complexes. Then, by adding NaBH(4) solution, Fe(3+) and GO are simultaneously reduced in situ to
Ja-An Annie Ho et al.
Analytical and bioanalytical chemistry, 391(2), 479-485 (2008-02-15)
Salmonellae are ubiquitous human pathogens, which pose a danger to the elderly and children. Due to the increased number of outbreaks of human illness associated with the consumption of contaminated products in the USA and many other countries, there is
Christos Haritoglou et al.
Retina (Philadelphia, Pa.), 29(9), 1266-1273 (2009-11-26)
The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human
A Arunarani et al.
Colloids and surfaces. B, Biointerfaces, 102, 379-384 (2012-09-27)
Basic Violet 3 and Acid Blue 93 are the most important group of synthetic colourants extensively used in textile industries for dyeing cotton, wool, silk and nylon. Release of these dye pollutants in to the environment adversely affects the human
Marie-Eve Asselin et al.
Pediatric dentistry, 30(1), 29-33 (2008-04-12)
The purpose of this in vitro study was to investigate and compare the microleakage-occurring after the placement of a light-curing sealant-to unground permanent enamel which had been previously conditioned using 3 different application protocols. Sixty-three molars were randomly distributed in
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