ROAMYGLL

Amyloglucosidase

from Aspergillus niger

• Synonyme(s) : Aspergillus niger, disaccharidase-type a-glucosidase
• Numéro de classification (Commission des enzymes): 3.2.1.3 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: Aspergillus niger
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate); ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
• Poids mol.: 97 kDa
• Conditionnement: pkg of 3,500 U (11202367001); pkg of 500 U (11202332001)
• Fabricant/nom de marque: Roche
• pH optimal: 4.6-4.8
• Température de stockage: 2-8°C

Description

Description générale

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase.[1] It is synthesized by several Aspergillus genus species.[2] This exo-enzyme is one of the major industrial enzymes.[3] The stability of amyloglucosidase can be increased by immobilization.[3]

Spécificité

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Application

Amyloglucosidase from Aspergillus niger is used for the determination of starch.[4]

Actions biochimiques/physiologiques

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose.[5][6] The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[7]

Notes préparatoires

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

• Western blot: 1 to 10 μg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash