11759779001
Roche
BM Chemiluminescence ELISA Substrate (AP)
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About This Item
Code UNSPSC :
12352204
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Utilisation
sufficient for 1,000 tests (ELISA)
sufficient for 600 tests (SEAP)
Conditionnement
pkg of 150 mL
Fabricant/nom de marque
Roche
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Spécificité
The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).
Caractéristiques et avantages
- Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
- High dynamic measuring range: Linear range of more than five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics
Composants
1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer
2. Enhancer, Emerald II
3. Assay Buffer
Principe
CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.
Notes préparatoires
Working solution: Preparation of working solution
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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