11719394001
Roche
Immunoprecipitation Kit (Protein A)
sufficient for 20 reactions, kit of 1, suitable for immunoprecipitation (IP)
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Utilisation
sufficient for 20 reactions
Conditionnement
kit of 1
Fabricant/nom de marque
Roche
Technique(s)
immunoprecipitation (IP): suitable
Température de stockage
2-8°C
Description générale
Immunoprecipitation is a widely used method for the analysis of target antigens in complex mixture of proteins. The protein of interest can be concentrated and immunoaffinity -purified in one step on an analytical scale via a specific antibody. Often, immunoprecipitated proteins are functionally fully active and can be further analyzed with respect to enzymatic activity, interactions, modifications and structure.
Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.
Application
Immunoprecipitation Kit (Protein A) has been used for the immunoprecipitation of proteins from cellular extracts with Protein A Agarose.
Conditionnement
1 kit containing 5 components.
Notes préparatoires
Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Composants de kit seuls
Réf. du produit
Description
- Core Buffer
- NaCl
- Detergent Mix
- cOmplete Protease Inhibitor Cocktail Tablets (5)
- Protein A Agarose ready-to-use
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
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Protocoles
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
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