10223581001
Roche
Poly(dA)
Poly-deoxy-adenylic acid
Synonyme(s) :
PCR
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About This Item
Code UNSPSC :
41105600
Produits recommandés
Forme
lyophilized
Poids mol.
330 Da
Conditionnement
pkg of 5 U
Fabricant/nom de marque
Roche
Rapport des absorbances
A290/260 nm 0.03-0.12
A280/260 nm 0.28-0.39
A250/260 nm 0.84-0.94
Température de stockage
−20°C (−15°C to −25°C)
Description générale
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
Application
Poly(dA) is suitable for use in:
- DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
- as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
- in Pyrococcus abyssi DNA polymerase assay
Séquence
Chain Length Mean chain length lot 20-17: 250-500 bases.
Définition de l'unité
1 A260 unit corresponds to 40 μg ssDNA.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
V Iyer et al.
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
Maga G, et al.
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G 40 P, interacts with forked DNA
Ayora S, et al.
Nucleic Acids Research, 30(11), 2280-2289 (2002)
A specific photoreaction in polydeoxyadenylic acid
Porschke D
Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)
A novel gene carrier prepared from triple helical beta-glucan and polydeoxyadenylic acid
Liu Q, et al.
Journal of materials chemistry. B, 2(8), 933-944 (2014)
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