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MABS21

Sigma-Aldrich

Anti-PORCN Antibody, clone 15G12.1

clone 15G12.1, from mouse

Synonyme(s) :

Probable protein-cysteine N-palmitoyltransferase porcupine, Protein MG61

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

15G12.1, monoclonal

Espèces réactives

rat, mouse, human

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgMκ

Numéro d'accès NCBI

NP_073736

Numéro d'accès UniProt

Q9H237

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Porcn(53627)

Catégories apparentées

Description générale

PORCN is a multi-pass endoplasmic reticulum protein involved in the processing of wingless and other Wnt proteins that facilitate their secretion from Wnt-producing cells. Studies have shown mutations in the genes that encode for PORCN cause a pleiotropic X-linked dominant disorder, focal dermal hypolasia (FDH, also known as Goltz syndrome). Skeletal abnormalities such as syndactyly, ectordactyly, and brachydactyly, and some cases of osteopathia striata have also been attibuted to PORCN abnormalities.

Immunogène

Linear peptide corresponding to human PORCN.

Application

Anti-PORCN Antibody, clone 15G12.1 is an antibody against PORCN for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PORCN in HeLa and A431 cells.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Qualité

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PORCN in 10 µg of mouse brain tissue lysate.

Description de la cible

~62 kDa observed.
Calculated molecular weight of 52 kDa The actual MW should be observed at ~45-65 kDa Helical membrane protein migration on SDS-PAGE may not exactly correlate with the calculated molecular weights (PNAS, 2009, 106:1760-1765).

Forme physique

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse brain tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Aritro Nath et al.
Scientific reports, 5, 14752-14752 (2015-10-02)
Hepatocellular carcinoma (HCC) is the second-leading cause of cancer-related death worldwide, and the factors influencing HCC progression are poorly understood. Here we reveal that HCC progression via induction of epithelial-mesenchymal transition (EMT) is closely associated with the expression of CD36/fatty
Cody J Aros et al.
Cell stem cell, 27(3), 413-429 (2020-07-30)
Our understanding of dynamic interactions between airway basal stem cells (ABSCs) and their signaling niches in homeostasis, injury, and aging remains elusive. Using transgenic mice and pharmacologic studies, we found that Wnt/β-catenin within ABSCs was essential for proliferation post-injury in vivo.

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