MABS1984
Anti-INTS1 Antibody, clone 4.47
clone 4.47, from mouse
Synonyme(s) :
Integrator complex subunit 1, Int1
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
4.47, monoclonal
Espèces réactives
human
Technique(s)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... INTS1(26173)
Description générale
Integrator complex subunit 1 (UniProt Q8N201; also known as Int1) is encoded by the INTS1 (also known as KIAA1440, UNQ1821/PRO3434) gene (Gene ID 26173) in human. INTS1 is a large protein that is a component of the integrator complex that is involved in small nuclear RNAs U1 and U2 transcription and their 3′-box-dependent processing. During transcriptional regulation and in RNA processing, it directly binds to the C-terminal domain of the RNA polymerase II largest subunit. It has been reported that INTS1 is also involved in different cell support processes, such as the formation of a scaffold for the assembly of integrator complex. Developing INTS1 (-/-) mouse embryos show growth arrest at the early blastocyst stage and exhibit activated caspase-3 and caspase-7 that induce apoptosis within the inner cell mass of blastocyst.
Ref.:
Baillat, D et al. (2005). Cell 123, 265-276.
Hata T., and Nakayama, M (2007), Biochim. Biophys. Acta 1773, 1039-1051.
Ref.:
Baillat, D et al. (2005). Cell 123, 265-276.
Hata T., and Nakayama, M (2007), Biochim. Biophys. Acta 1773, 1039-1051.
Spécificité
Clone 4.47 targets an epitope in the central region of human INTS1 protein.
Immunogène
GST-tagged recombinant human INTS1 internal fragment.
Application
Anti-INTS1, clone 4.47 Antibody, Cat. No. MABS1984, is a highly specific mouse monoclonal antibody, that targets INTS1 and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualité
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected in 50 µg of HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected in 50 µg of HeLa cell lysate.
Description de la cible
~240 kDa observed. 244.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Felipe Beckedorff et al.
Cell reports, 32(3), 107917-107917 (2020-07-23)
Transcription by RNA polymerase II (RNAPII) is pervasive in the human genome. However, the mechanisms controlling transcription at promoters and enhancers remain enigmatic. Here, we demonstrate that Integrator subunit 11 (INTS11), the catalytic subunit of the Integrator complex, regulates transcription
Stephin J Vervoort et al.
Cell, 184(12), 3143-3162 (2021-05-19)
Gene expression by RNA polymerase II (RNAPII) is tightly controlled by cyclin-dependent kinases (CDKs) at discrete checkpoints during the transcription cycle. The pausing checkpoint following transcription initiation is primarily controlled by CDK9. We discovered that CDK9-mediated, RNAPII-driven transcription is functionally
Jasmine Barra et al.
Science advances, 6(27), eaaz9072-eaaz9072 (2020-09-15)
RNA 3' end processing provides a source of transcriptome diversification which affects various (patho)-physiological processes. A prime example is the transcript isoform switch that leads to the read-through expression of the long non-coding RNA NEAT1_2, at the expense of the
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique