MABS195
Anti-Pan-Ras Antibody, clone RAS 10
clone RAS 10, from mouse
Synonyme(s) :
GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase Kras, N-terminally processed
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
RAS 10, monoclonal
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRAS(3845)
Description générale
The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
Immunogène
Recombinant protein corresponding to human Pan-Ras.
Application
Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Qualité
Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Description de la cible
~21 kDa observed
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
MCF7 cell lysate
MCF7 cell lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Won-Ji Ryu et al.
Experimental & molecular medicine, 52(5), 832-842 (2020-05-28)
Triple-negative breast cancer (TNBC) is a severe and heterogeneous disease that lacks an approved targeted therapy and has a poor clinical outcome to chemotherapy. Although the RAS-ERK signaling axis is rarely mutated in TNBC, ~50% of TNBCs show an increased
F Roßner et al.
Oncogenesis, 5, e187-e187 (2016-01-19)
The transcription factor YBX1 can act as a mediator of signals transmitted via the EGFR-RAS-MAPK axis. YBX1 expression has been associated with tumor progression and prognosis in multiple types of cancer. Immunohistochemical studies have revealed dependency between YBX1 expression and
Sang-Kyu Lee et al.
EMBO reports, 19(12) (2018-11-11)
RAS proteins play critical roles in various cellular processes, including growth and transformation. RAS proteins are subjected to protein stability regulation via the Wnt/β-catenin pathway, and glycogen synthase kinase 3 beta (GSK3β) is a key player for the phosphorylation-dependent RAS
Alisa Damnernsawad et al.
Haematologica, 107(1), 77-85 (2020-12-31)
Drug resistance impedes the long-term effect of targeted therapies in acute myeloid leukemia (AML), necessitating the identification of mechanisms underlying resistance. Approximately 25% of AML patients carry FLT3 mutations and develop post-treatment insensitivity to FLT3 inhibitors, including sorafenib. Using a
Won-Ji Ryu et al.
Cancers, 11(4) (2019-04-11)
: Treatment of advanced gastric cancer patients with current standard chemotherapeutic agents frequently results in resistance, leading to poor overall survival. However, there has been no success in developing strategies to overcome it. We showed the expression levels of both
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