MABN856
Anti-Neurophysin 2/NP-AVP Antibody, clone PS 45
clone PS 45, from mouse
Synonyme(s) :
Vasopressin-neurophysin 2-copeptin, AVP-NPII, Neurophysin 2, Neurophysin-II
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
PS 45, monoclonal
Espèces réactives
mouse, human, bovine, frog, rat
Technique(s)
ELISA: suitable
electron microscopy: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
Isotype
IgG2bκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Avp(24221)
Description générale
The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormons oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormon peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Posttranslational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an antidiuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
Spécificité
Clone PS 45 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.
Immunogène
Epitope: Near Neurophysin 2 C-terminus
Application
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat brain and rat pituitary tissue.
Radioimmunoassay (RIA): Target specificity of a representative lot was confirmed by RIA. Only rat neurophysin 2, but not neurophysin 1, oxytocin, or angiotensin II, can compete against 125I-neurophysin 2 for binding mAb PS 45 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy (EM): A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
3. References/ Please add reference:
Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97
Radioimmunoassay (RIA): Target specificity of a representative lot was confirmed by RIA. Only rat neurophysin 2, but not neurophysin 1, oxytocin, or angiotensin II, can compete against 125I-neurophysin 2 for binding mAb PS 45 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy (EM): A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
3. References/ Please add reference:
Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97
This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 45 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Radioimmunoassay, Electron Microscopy, Immunoprecipitation, ELISA for the detection of Neurophysin 2/NP-AVP.
Qualité
Evaluated by Western Blotting in rat pituitary tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Description de la cible
~15 kDa observed
Forme physique
Format: Purified
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Iwona Olejniczak et al.
Frontiers in physiology, 13, 909795-909795 (2022-10-25)
Searching for food follows a well-organized decision process in mammals to take up food only if necessary. Moreover, scavenging is preferred during their activity phase. Various time-dependent regulatory processes have been identified originating from the suprachiasmatic nuclei (SCN), which convert
Juan Pablo Arroyo et al.
JCI insight, 7(24) (2022-11-04)
Vasopressin has traditionally been thought to be produced by the neurohypophyseal system and then released into the circulation where it regulates water homeostasis. The questions of whether vasopressin could be produced outside of the brain and if the kidney could
Stefano Musardo et al.
eLife, 11 (2022-04-23)
Social interaction during adolescence strongly influences brain function and behavior, and the recent pandemic has emphasized the devastating effect of social distancing on mental health. While accumulating evidence has shown the importance of the reward system in encoding specific aspects
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