MABE463M

Anti-methylated TLS/FUS Antibody, clone 9G6

clone 9G6, from rat

• Synonyme(s) : RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 9G6, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_001164105
• Numéro d'accès UniProt: P35637
• Modification post-traductionnelle de la cible: methylation (not specified)
• Informations sur le gène: human ... FUS(2521)

Description

Description générale

RNA-binding protein FUS (UniProt: also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It is involved in a variety of cellular functions including transcription, protein translation, RNA splicing, and transport and may play a role in maintenance of genomic integrity. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).

Spécificité

Clone 9G6 detects methylated RNA-binding protein FUS in human cells. It targets an epitope with in 31 amino acids from the C-terminal region.

Immunogène

Ovalbumin-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region corresponding to the RGG3 domain of FUS (with all arginines methylated).

Application

Anti-methylated TLS/FUS, clone 9G6, Cat. No. MABE463, is a highly specific rat monoclonal antibody that targets methylated RNA-binding protein FUS and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of HeLa cell lysate.

Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of lysate from HeLa cells pretreated with AdOx. (Dormann, D., et al. (2012). The EMBO J. 31(22):4258-75)

Western Blotting Analysis: A representative lot detected methylated TLS/FUS in Western Blotting applications (Suárez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).

Qualité

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.05 ug/mL of this antibody detected methylated TLS/FUS in 10 µg of HepG2 cell lysate.

Description de la cible

~70 kDa observed; 53.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash