MABE2022
Anti-GINS2/PSF2 Antibody, clone 78C
Synonyme(s) :
DNA replication complex GINS protein PSF2;GINS complex subunit 2
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
78C, monoclonal
Poids mol.
calculated mol wt 21.43 kDa
Produit purifié par
using protein G
Espèces réactives
human
Conditionnement
antibody small pack of 100 μg
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG2a
Séquence de l'épitope
Unknown
Numéro d'accès Protein ID
Numéro d'accès UniProt
Conditions d'expédition
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GINS2(51659)
Description générale
DNA replication complex GINS protein PSF2 (UniProt: Q9Y248; also known as GINS complex subunit 2) is encoded by the GINS2 (also known as PSF2, CGI-122, DC5, HSPC037) gene (Gene ID: 51659) in human. GINS/PSF2 is a component of the GINS complex, which is a heterotetramer of GINS 1, 2, 3, and 4 that are assembled with 1:1:1:1 stoichiometry. Each of these four subunits is essential for proper completion of DNA replication initiation and elongation. This complex plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex binds preferentially to single-stranded DNA. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein Cdc45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. GINS2 gene is reported to be frequently up-regulated in the cancer cells. Transfection of ETK-1 and HuH28 cells with PSF2 siRNA is shown to reduce the amount of transcript and suppress cell growth, indicating the important role of GINS2/PF2 in development of cholangiocarcinoma. (Ref.: Aparicio, T., et al. (2009). Nucl. Acids Res. 37(7); 2087-2095; Boskovic, J., et al. (2007). EMBO Rep. 8(7); 678-684; Obama, K., et al. (2005). Oncol. Rep. 14(3):701-706).
Spécificité
Clone 78C is a mouse monoclonal antibody that detects human DNA replication complex GINS protein PSF2 (GINS2/PSF2).
Immunogène
Recombinant human GINS2/PSF2 expressed in E. coli.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.
Tested Applications
Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.
Tested Applications
Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Recommended storage: +2°C to +8°C.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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