MABE1805
Anti-Rabex-5 Antibody, clone G32
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
G32, monoclonal
Poids mol.
calculated mol wt 56.89 kDa
observed mol wt ~57 kDa
Produit purifié par
using protein G
Espèces réactives
human
Conditionnement
antibody small pack of 100 μg
Technique(s)
western blot: suitable
Isotype
IgG2aκ
Séquence de l'épitope
N-terminal
Numéro d'accès Protein ID
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RABGEF1(2742)
Description générale
Rab5 GDP/GTP exchange factor (UniProt: Q9UJ41; also known as RAP1, Rabaptin-5-associated exchange factor for Rab5, Rabex-5) is encoded by the RABGEF1 (also known as RABEX5) gene (Gene ID: 27342) in human. Rabex-5 is a guanine nucleotide exchange factor that catalyzes the exchange of GTP for GDP on the Rab5 subfamily of low molecular weight GTPases. It forms a tight complex with Rabaptin-5 that augments its exchange activity and generates GTP-bound, membrane-associated Rab5, which in turn binds Rabaptin-5 and stabilizes the Rabex-5- Rabaptin-5 complex on endosomes. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It can also act as a ubiquitin ligase. It binds to ubiquitin (Ub) through two independent ubiquitin-binding domains (UBDs). These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated epidermal growth factor receptors. The first Ub binding region is localized in amino acids 2-49 and is named as RUZ domain (Rabex-5 ubiquitin binding zinc finger). The second Ub binding region is highly conserved and is localized in amino acids 48-74. In this domain Ala58 is shown to be critical for Ub binding and A58G mutagenesis causes significant reduction in its binding to Ub. (Ref.; Penengo, L., et al. (2006). Cell. 124. 1183-1195; Mattera, R., et al. (2006). J. Biol. Chem. 281(10); 6874-6883).
Spécificité
Clone G32 is a mouse monoclonal antibody that detects Rabex-5. It targets an epitope within 49 amino acids from the N-terminal region.
Immunogène
GST-tagged full length recombinant human Rabex-5.
Application
Quality Control Testing
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rabex-5, clone G32, Cat. No. MABE1805, is a mouse monoclonal antibody that detects Rebex-5 and is tested for use in Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique