MABC1736

Anti-CIDE-A Antibody, clone V62P1E3 B10

• Synonyme(s) : Cell death activator CIDE-A;Cell death-inducing DFFA-like effector A
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: V62P1E3 B10, monoclonal
• Poids mol.: calculated mol wt 24.69 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_001270
• Numéro d'accès UniProt: O60543
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CIDEA(1149)

Description

Description générale

Cell death activator CIDE-A (UniProt: O60543; also known as Cell death-inducing DFFA-like effector A) is encoded by the CIDEA gene (Gene ID: 1149) in human. Three members of the CIDE family of proteins have been described in human CIDE-A, CIDE-B, and CIDE-C. They all contain a common N-terminal CIDE-N domain and a C-terminal CIDE-C domain. CIDE-A is a brown fat-associated protein that has been implicated in lipid and energy metabolism. Its expression has been reported in colon and normal breast tissue. It binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. It is shown to be enriched at lipid droplet contact sites and at focal contact sites between lipid droplets where it promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. It is reported to strongly promote triglyceride accumulation even in cell types that do not normally store large amounts of neutral lipid. Its expression levels in adipose tissue have been correlated with insulin sensitivity, which could be related to enhanced storage of triglyceride resulting in reduced levels of fatty acid levels in the circulation. CIDE-A is also reported to acts as a CCAAT Enhancer Binding Protein b (CEBPB) coactivator in mammary epithelial cells and controls the expression of CEBPB downstream target genes, such as ID2, IGF1, PRLR, SOCS1, SOCS3, XDH. Higher expression of CIDE-A has been reported in colorectal tumors compared to normal colonic mucosa. (Ref.: Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147; Puri, V., et al. (2008). Proc. Natl. Acad. Sci. USA. 105(22); 7833-7838).

Spécificité

Clone V62P1E3 B10 is a mouse monoclonal antibody that detects human Cell death activator CIDE-A. It targets an epitope within 9 amino acids from the C-terminal region.

Immunogène

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human CIDE-A.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human colon cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CIDE-A in human colon cancer tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected CIDE-A in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

ELISA Analysis: A representative lot detected CIDEA in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry Applications: A representative lot detected CIDEA in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CIDE-A, clone V62P1E3 B10, Cat. No. MABC1736, is a mouse monoclonal antibody that detects CIDE-A and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable