MABC1147

Anti-CIP2A Antibody, clone 6G8-G1

clone 6G8-G1, from mouse

• Synonyme(s) : Protein CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6G8-G1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_065941
• Numéro d'accès UniProt: Q8TCG1
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KIAA1524(57650)

Description

Description générale

Protein CIP2A (UniProt: Q8TCG1; also known as CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen) is encoded by the KIAA1524 (also known as CIP2A) gene (Gene ID: 57650) in human. CIP2A is a single pass membrane protein that exhibits dynamic changes in localization, including the cytoplasm and centrosome, depending on the stage of the cell cycle. It is expressed at low levels in most tissues, however, it is overexpressed in several cancers, including head-and-neck squamous cell carcinomas and liver cancer cells. Levels of CIP2A are correlated with the proliferation of human cancer cells and with cancer progression. It acts as an oncoprotein and inhibits protein phosphatase 2A activity and thereby stabilizes c-Myc in human malignancies. It promotes anchorage-independent cell growth and tumor formation. Higher activity of c-Myc rapidly induces the activation of cyclin E-cdk2 kinase activity. Concurrently, the release of p27Kip1 from Cdk2 complexes enhances cell proliferation. In CIP2A depleted cells, centrosome separation and the mitotic spindle dynamics were impaired, which leads to the activation of spindle assembly checkpoint signaling and, ultimately, delayed cell division time. CIP2A is also reported to interact with NIMA (never in mitosis gene A)-related kinase 2 (NEK2) during G2/M phase and enhances NEK2 kinase activity that facilitates centrosome separation in a PP1- and PP2A-independent manner. (Ref.: Jeong, AL et al. (2014). J. Biol. Chem. 289(1): 28-40).

Spécificité

Clone 6G8-G1 specifically detects CIP2A in HEK293 cells overexpressing CIP2A.

Immunogène

Epitope: N-terminus

His-tagged recombinant fragment from the N-terminal region of human CIP2A.

Application

Detect CIP2A using this mouse monoclonal Anti-CIP2A Antibody, clone 6G8-G1, Cat. No. MABC1147, tested in Western Blotting.

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in HEK293 cells overexpressing CIP2A.

Western Blotting Analysis: 1 µg/mL of this antibody detected CIP2A in 10 µg of lysate from HEK293 cells overexpressing CIP2A.

Description de la cible

~102 kDa observed; 102.18 calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1