MAB1285
Anti-HLA-B27 Antibody, clone HLA.ABC.m3
clone HLA.ABC.m3, Chemicon®, from mouse
Synonyme(s) :
Anti-AS, Anti-B-4901, Anti-HLAB
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
HLA.ABC.m3, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... HLA-B(3106)
Catégories apparentées
Description générale
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
Spécificité
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
Application
This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
Forme physique
Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
Stockage et stabilité
Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Barbara Neerinckx et al.
RMD open, 3(1), e000451-e000451 (2017-09-08)
The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis
Christoffer Dellgren et al.
Journal of immunology (Baltimore, Md. : 1950), 197(12), 4807-4816 (2016-11-09)
HLA class I cell surface expression is crucial for normal immune responses, and variability in HLA expression may influence the course of infections. We have previously shown that classical HLA class I expression on many human cell types is biased
Shengli Song et al.
Frontiers in immunology, 12, 705140-705140 (2021-07-31)
Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major histocompatibility complex (MHC) mismatches are especially problematic. Most research directed
Congjing An et al.
Cancer cell international, 22(1), 184-184 (2022-05-14)
Breast cancer (BC) is one of the most prevalent malignancies among women globally. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are associated with BC carcinogenesis. In the current study, we explored the mechanism by which LINC00662 regulates BC. Quantitative
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