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MAB1061

Sigma-Aldrich

Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2

clone ID1.2, Chemicon®, from mouse

Synonyme(s) :

BSPII, Integrin-binding Sialoprotein

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

ID1.2, monoclonal

Espèces réactives

human

Ne doit pas réagir avec

bovine, rat, pig

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... IBSP(3381)
pig ... Ibsp(397137)
rat ... Ibsp(24477)

Description générale

Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.

Spécificité

Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.

Immunogène

Bone Sialoprotein isolated from human bones.
Epitope: C-terminus

Application

Research Category
Cell Structure
Research Sub Category
Osteobiology
This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.
Western blot: (1μg/mL)

Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)

Immunocytochemistry: (1:100)

ELISA: (1μg/mL)

RIA

Optimal working dilutions must be determined by the end user.

Liaison

Replaces: AB1855

Forme physique

Format: Purified
Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.

Stockage et stabilité

Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Liisa T Kuhn et al.
Tissue engineering. Part A, 20(1-2), 365-377 (2013-08-21)
The in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem cells (hESC-MCs) was evaluated in vivo by implantation on collagen/hydroxyapatite scaffolds into calvarial defects in immunodeficient mice. This study is novel because no osteogenic or chondrogenic differentiation
Hye Jin Kim et al.
Theranostics, 12(14), 6409-6421 (2022-09-29)
The efficiency of gene therapy is often dictated by the gene delivery system. Cationic polymers are essential elements of gene delivery systems. The relatively cheap cationic polymer, polyethyleneimine, has high gene delivery efficiency and is often used for gene delivery.
Xiaonan Xin et al.
Stem cells translational medicine, 3(10), 1125-1137 (2014-08-15)
The use of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) for study and treatment of bone diseases or traumatic bone injuries requires efficient protocols to differentiate hESCs/iPSCs into cells with osteogenic potential and the ability to
Xiaonan Xin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 67(6), 401-417 (2019-03-09)
The effectiveness of autologous cell-based skeletal repair continues to be controversial in part because in vitro predictors of in vivo human bone formation by cultured human progenitor cells are not reliable. To assist in the development of in vivo assays

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