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DR1002

Sigma-Aldrich

PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

liquid, clone 10H11.E12, Calbiochem®

Synonyme(s) :

Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

Type de produit anticorps

primary antibodies

Clone

10H11.E12, monoclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

human, mouse

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Protein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser1981.
Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
This PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

Immunogène

Human
a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM

Application





Immunoblotting (0.5 g/ml)
Immunocytochemistry (1.5 g/ml)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Irritant (B)

Forme physique

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
HeLa cells treated with camptothecin (Cat. No. 208925)

Autres remarques

Does not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Horejsi, Z., et al. 2004. Oncogene23, 3122.
Abraham, R.T. 2003. Genes Dev.15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer3, 155.

Informations légales

Sold under license of U.S. Patent 7,108,992.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Hongyu Chen et al.
Journal of cellular physiology, 234(7), 11871-11881 (2018-11-28)
Acquired resistance to cytotoxic antineoplastic agents is a major clinical challenge in tumor therapy; however, the mechanisms involved are still poorly understood. In this study, we show that knockdown of CtIP, a corepressor of CtBP, promotes cell proliferation and alleviates
Xiaoyao Yang et al.
International journal of molecular medicine, 42(4), 1945-1954 (2018-08-08)
Utilizing the energy released from the nuclear fusion of deuterium with tritium (D‑T) may be an important method of supplying energy in the future. The ionizing radiation emitted from nuclear fusion is a potential health risk to humans, including scientists
Wissam Assaily et al.
Molecular cell, 44(3), 491-501 (2011-11-08)
The p53 protein is activated by stress signals and exhibits both protective and death-promoting functions that are considered important for its tumor suppressor function. Emerging evidence points toward an additional role for p53 in metabolism. Here, we identify Lpin1 as
Tatyana V Pospelova et al.
Cell cycle (Georgetown, Tex.), 8(24), 4112-4118 (2009-12-01)
Cellular senescence is currently viewed as a response to DNA damage. In this report, we showed that non-damaging agents such as sodium butyrate-induced p21 and ectopic expression of either p21 or p16 cause cellular senescence without detectable DNA breaks. Nevertheless
Nibedita Patel et al.
Scientific reports, 7(1), 4263-4263 (2017-06-29)
The B-lymphoma Moloney murine leukemia virus insertion region-1 protein (BMI1) acts as an oncogene in various cancers, including breast cancer. Recent evidence suggests that BMI1 is rapidly recruited to sites of DNA double strand breaks where it facilitates histone H2A

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