ABT340

Anti-AES Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Amino-terminal enhancer of split, GRG, ESP1, TLE5, Gp130-associated protein GAM, Protein ESP1, Protein GRG
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Numéro d'accès UniProt: Q08117
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TLE5(166)

Description

Description générale

Amino-terminal enhancer of split (AES), also known as GRG or ESP1 or TLE5 or Gp130-associated protein GAM, is a member of the Groucho/ transducin-like enhancer of split (TLE) family. AES belongs to a group of transcriptional co-repressors that play an important role in neurogenesis, segmentation and sex determination. AES is suggested to be a dominant repressor towards other family members of the TLE family and might be required for the initiation and maintenance of the differentiated state. AES has been shown to inhibit the NF-kappa-B-regulated gene expression. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1). Recent studies suggest that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. The apoptosis of cells transfected with AES and Bit1 implies that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and more specifically – with the Alpha5Beta1 integrin. AES is found predominantly in muscle, heart and placenta. Additionally, in fetal tissues, AES is expressed highly in the heart, lung, kidney, brain and liver.

Spécificité

This antibody recognizes the C-terminus of AES.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human AES.

Application

Anti-AES Antibody is a highly specific rabbit polyclonal antibody, that targets Amino-terminal enhancer of split & has been tested in western blotting, ICC & Immunofluorescence.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected AES in HeLa cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected AES in HeLa cells.

Research Category
Cell Structure

Cell Structure

Research Sub Category
Adhesion (CAMs)

Qualité

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected AES in 15 µg of HEK293 cell lysate.

Description de la cible

~22 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~22 kDa and ~29 kDa

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HEK293 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable