ABS2248

Anti-OVCH2

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: calculated mol wt 67.61 kDa; observed mol wt ~74 kDa
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 100 μL
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès UniProt: Q7M761
• Conditions d'expédition: dry ice
• Température de stockage: -10 to -25°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Ovch2(244199)

Description

Description générale

Ovochymase-2 (UniProt: Q7M761; Oviductin, OVCH2) is encoded by the Ovch2 (also known as Ovtn) gene (Gene ID: 244199) in murine species. OVCH2 is a member of the peptidase S1 family that is synthesized with a signal peptide (aa 1-22) that is subsequently cleaved off. It also has a propeptide (aa 23-51) that serves as an activation peptide. OVCH2 is mainly localized in the caput region of epididymis that is most proximal to the testis. It is shown to be essential for processing ADAM3, the sperm surface protein that is required for the fertilizing ability of sperm. It is reported that a neural epidermal growth factor like like 2 (NELL2), binds to an orphan receptor tyrosine kinase, c-Ros oncogene 1 (ROS1), and mediates the differentiation of the initial segment (IS) of the caput epididymis. Male mice with NELL2 are reported to be infertile. (Ref.: Kiyozumi, D., et al. (2020). Science. 368(6495); 1132-1135).

Spécificité

This rabbit polyclonal antibody detects murine Ovochymase-2 (OVCH2). It targets an epitope within the CUB2 domain from the C-terminal region.

Immunogène

GST-tagged recombinant fragment corresponding to 179 amino acids from the C-terminal region of murine Ovochymase-2 (OVCH2).

Application

Quality Control Testing

Evaluated by Western Blotting in mature mouse caput epididymis lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OVCH2 in mature mouse caput epididymis lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected OVCH2 in mature mouse caput epididymis lysate (Courtesy of Dr. Masahito Ikawa Lab @ Osaka University, Japan).

Western Blotting Analysis: A representative lot detected OVCH2 in Western Blotting applications (Kiyozumi, D., et. al. (2020). Science. 368(6495):1132-1135).

Immunohistochemistry Applications: A representative lot detected OVCH2 in Immunohistochemistry applications (Kiyozumi, D., et. al. (2020). Science. 368(6495):1132-1135).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

This rabbit polyclonal Anti-OVCH2, Cat. No. ABS2248, is a rabbit polyclonal antibody that detects Ovochymase-2 (OVCH2) and is tested for use in Immunohistochemistry and Western Blotting.

Forme physique

Rabbit polyclonal antibody in 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable