ABE605

Anti-TREX1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Three prime repair exonuclease 1, CRV, AGS1, DRN3, HERNS, 3′-5′ exonuclease TREX1, DNase III
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès UniProt: Q9NSU2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TREX1(11277)

Description

Description générale

TREX1, (DNase III), or three prime repair exonuclease 1 is the major 3’→ 5’ DNA exonuclease in mammalian cells. TREX1 binds preferentially to ssDNA and can also remove mismatched 3’ terminal deoxyribonucleotides at DNA strand breaks. The protein is required for proper DNA checkpoint signaling and plays critical roles in DNA replication and gap filling during DNA repair. TREX1 has a perinuclear distribution in mammalian cells and is colocalized with ER-associated proteins in cells at rest. TREX1 relocalizes to the nucleus and specifically to replication forks during S phase and periods of DNA synthesis and repair such as after irradiation. TREX1 acts on an ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates and thus acts to attenuate DNA damage checkpoint signaling and prevent pathological immune activation in normal cells. Defects in TREX1 protein have been associated in humans with many inherited diseases including Aicardi-Goutiėres syndrome type 1 (AGS1), systemic lupus erythematosus (SLE), chilblain lupus type 1 (CHBL1), and vasculopathy, retinal, with cerebral leukodystrophy (RVCL). EMD Millipore’s Anti-TREX1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human samples.

Immunogène

KLH-conjugated linear peptide corresponding to human TREX1.

Application

Detect DNASE using this rabbit polyclonal antibody, Anti-TREX1 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected TREX1 in human spleen tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected TREX1 in human spleen tissue.

Qualité

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TREX1 in 15 µg of human spleen tissue lysate.

Description de la cible

~30 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~32 kDa, ~33 kDa, and ~39 kDa

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable