Mammalian homolog of drosophila flightless-1 is encoded by the FLII gene in human (also known as FLI, Fli1, or FLIL). The human flightless-1 homolog (Flii) contains sixteen 23-amino acid leucine-rich repeats (LRR) in its N-terminal half and a gelsolin-like domain (GLD) in its C-terminal half, composed of six segmental repeats typical of many gelsolin family members. Flii is reported to interact with actin and "FLI LRR associated protein" (FLAP) via its GLD and LRR domain, respectively (PMID 9525888). Flii is also shown to interact with the glucose-responsive transcription factor carbohydrate responsive element binding protein (ChREBP) via both the LRR and GLD domains and functions as a negative regulator of ChREBP in cancer cells (PMID 24055811). Increased Flii level is observed in the blistered skin of patients with epidermolysis bullosa acquisita (EBA), and Flii overexpression in transgenic mice results in delayed epidermal barrier formation in embryos and decreased expression of tight junction (TJ) proteins, as well as impaired barrier function among adult transgenic mice (PMID 24375017).
Immunogène
KLH-conjugated linear peptide corresponding to human Flightless-1 homolog.
Application
Detect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot of this antibody detected Flightless-1 homolog in IP (Prof. D. Pallas, Emory University).
Qualité
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flightless-1 homolog in 10 µg of A431 cell lysate.
Description de la cible
~144 kDa observed
Liaison
Replaces: MABF195; MABE530
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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