ABE1845

Anti-GABPA Antibody

from rabbit

• Synonyme(s) : GA-binding protein alpha chain, GABP subunit alpha, GABPA
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, rat, mouse
• Réactivité de l'espèce (prédite par homologie): monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
• Technique(s): ChIP: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_032091
• Numéro d'accès UniProt: Q00422
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GABPA(2551) ; mouse ... Gabpa(14390)

Description

Description générale

GA-binding protein alpha chain (UniProt Q00422; also known as GA repeat binding protein alpha, GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor-2) is encoded by the Gabpa gene (also known as E4tf1a) in murine species (Gene ID 14390). GABPalpha is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPalpha is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Immunogène

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to mouse GABPA near the C-terminus.

Application

Detect GABPA using this Anti-GABPA Antibody validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected GABPA in mouse liver nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Chromatin Immunoprecipitation Analysis: A representative lot detected GABPA occupancy at the Sod2 promoter region by ChIP using liver chromatin preparation from mice after S. aureus inoculation (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).

Qualité

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABPA in 10 µg of mouse liver tissue lysate.

Description de la cible

~55 kDa observed

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable