ABE1342

Anti-ZBTB4 (KAISO-L1)

from rabbit

• Synonyme(s) : Zinc finger and BTB domain-containing protein 4, KAISO-like zinc finger protein 1, KAISO-L1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_001122305
• Numéro d'accès UniProt: Q9P1Z0
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ZBTB4(57659)

Description

Description générale

Zinc finger and BTB domain-containing protein 4 (UniProt: Q9P1Z0; also known as KAISO-like zinc finger protein 1, KAISO-L1, ZBTB4) is encoded by the ZBTB4 (also known as KIAA1538) gene (Gene ID: 57659) in human. ZBTB4 is an epigenetic regulator that localizes at pericentromeric heterochromatin. It is shown to be an essential component in maintaining genomic stability in mammals. It serves as a transcriptional repressor with bimodal DNA-binding specificity. It represses transcription in a methyl-CpG-dependent manner and binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5′-CGCG-3′, but can also bind to the non-methylated consensus sequence 5′-CTGCNA-3′ (also known as the consensus kaiso binding site). ZBTB4 contains six C2H2-type zinc-finger domains and two proline-rich regions (aa 435-536 and 610-654). Its BTB (Broad-Complex, Tramtrack and Bric a brac) domain is localized in the amino acids 30-152. It is shown to be is frequently downregulated in cancer. ZBTB4 is phosphorylated by HIPK2 (Homeodomain Interacting Protein Kinase 2) and phosphorylation reduces its stability and triggers its degradation in response to DNA damage. (Ref.: Roussel-Gervais A., et al. (2017). Cancer Res; 77(1); 62-73; Yamada, D., et al (2009). Oncogene. 28(27); 2535-2544).

Spécificité

This rabbit polyclonal antibody detects Zinc finger and BTB domain-containing protein 4 (KAISO-L1) in human cells. It targets an epitope within 17 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human Zinc finger and BTB domain-containing protein 4.

Application

Anti-ZBTB4 (KAISO-L1), Cat. No. ABE1342, is a highly specific rabbit polyclonal antibody that targets Zinc finger and BTB domain-containing protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ZBTB4 (KAISO-L1) in human fallopian tube and human cervical cancer tissue sections.

Research Category
Epigenetics & Nuclear Function

Qualité

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ZBTB4 (KAISO-L1) in PC3 cell lysate.

Description de la cible

~105 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1