ABE1310

Anti-ALKBH1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_006011
• Numéro d'accès UniProt: Q13686
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ALKBH1(8846)

Description

Description générale

ALKBH1 is a DNA lyase that is critical for DNA repair and specifically repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. ALKBH1 can also cleave both single-stranded and double-stranded DNA at basic sites, and ALKBH1 can induce double stranded breaks at basic sites. Finally, ALKBH1 plays a role in placental trophoblast lineage differentiation during development, and ALKBH1 may also play a role in neuronal development and migration. ALKBH1 is widely expressed and localizes to mitochondria and nucleus. The nuclear localization of ALKBH1 appears to be as part of fusion proteins with nuclear targeted proteins or when the first 26 N-terminal residues are removed.

Spécificité

Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA (Westbye, M.P., et al. (2008). JBC. 283:25046-25056).

Immunogène

Epitope: Around the Substrate binding region

KLH-conjugated linear peptide corresponding to the substrate binding region of human ALKBH1.

Application

Anti-ALKBH1 Antibody is an antibody against ALKBH1 for use in Immunohistochemistry and Immunocytochemistry.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ALKBH1 in human pancreas and human kidney tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Qualité

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected ALKBH1 in 10 µg of human skeletal muscle tissue lysate.

Description de la cible

~45 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable