AB9985

Anti-glutathione p53 (Cys141) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53 antigen, p53 transformation suppressor, p53 tumor suppressor, transformation-related protein 53, tumor protein p53
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey (100% sequence homology), rat (92% sequence homology), chimpanzee (100% sequence homology), mouse (85% sequence homology), orangutan (100% sequence homology), rabbit (100% sequence homology), ground squirrel (100% sequence homology), rhesus macaque (100% sequence homology), hamster (100% sequence homology), marmoset (100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_000537
• Numéro d'accès UniProt: P04637
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: glutathionylation (Cys141)
• Informations sur le gène: human ... TP53(7157)

Description

Description générale

Cellular tumor antigen p53 is a member of the p53 family and is found in the cytoplasm, nucleus, and endoplasmic reticulum. It functions as a tumor suppressor within a variety of tumors by either stimulating apoptosis or growth arrest in deference to cell type and physiological factors. p53 serves as a trans-activator, negatively regulating cell division during cell cycle regulation. It is also thought to be involved in the cross-over for Notch signaling. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, esophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortival carcinoma. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function, and b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

Spécificité

This antibody recognizes p53 when glutathionylated at Cys141.

Immunogène

Epitope: p53 glutathionylated at Cys141

KLH-conjugated linear peptide corresponding to human p53 glutathionylated at Cys141.

Application

Anti-glutathione p53 (Cys141) Antibody is a rabbit polyclonal antibody for detection of glutathione p53 (Cys141) also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53 & has been validated in WB, IP, IHC, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Dr. Kalkunte S. Srivenugopal, Department of Biomedical Sciences, Texas Tech University Health Sciences Center, 1406 S. Coulter, Amarillo, TX 79106.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Yusef, M, et al. (2010). Free Radic Biol Med. 49(5):908-917.)

Qualité

Evaluated by Immunohistochemistry in prostate adenocarcinoma tissue.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected glutathione p53 in prostate adenocarcinoma tissue.

Description de la cible

~53 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Prostate adenocarcinoma tissue

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable