AB9856

Anti-TorsinB Antibody

serum, Chemicon^®

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: rat, mouse, human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NM_014506.1
• Numéro d'accès UniProt: O14657
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TOR1B(27348)

Description

Spécificité

TorsinB. The antibody reacts with the ~38 kDa protein.

Immunogène

Synthetic peptide from torsinB.

Application

Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC.

Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.

Optimal working dilutions must be determined by end user.

Stockage et stabilité

Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable