AB3573

Anti-BCL-XL (pS62) Antibody

Chemicon^®, from rabbit

• Synonyme(s) : Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat (100% homologous), mouse (100% homologous)
• Fabricant/nom de marque: Chemicon^®
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NM_004322.2; NM_032989.1
• Numéro d'accès UniProt: Q92934
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer62)
• Informations sur le gène: human ... BCL2L1(598)

Description

Description générale

Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.

Spécificité

Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react.

Immunogène

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat.

Application

Anti-BCL-XL (pS62) Antibody detects level of BCL-XL (pS62) & has been published & validated for use in WB.

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Qualité

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62.

Liaison

Replaces: 04-432

Forme physique

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.

Stockage et stabilité

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Remarque sur l'analyse

Control
SK-BR-3 cells treated with vinblastine.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2