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AB1926

Sigma-Aldrich

Anti-Integrin β5 Antibody, CT/cytosolic

serum, Chemicon®

Synonyme(s) :

Anti-Anti-AA475909, Anti-Anti-AI874634, Anti-Anti-ESTM23, Anti-Anti-[b]-5, Anti-Anti-[b]5, Anti-Anti-[b]5A, Anti-Anti-[b]5B, Anti-Anti-beta-5, Anti-Anti-beta5

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

mouse, hamster, human, rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

Numéro d'accès NCBI

NM_002213.3

Numéro d'accès UniProt

P18084

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ITGB5(3693)
mouse ... Itgb5(16419)
rat ... Itgb5(257645)

Description générale

The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.

The beta5 subunit associates with alphaV forming a receptor for vitronectin. This complex is expressed in a wide range of cell types including fibroblast and keratinocytes. It is also expressed at high levels in several tumors including lung, pancreatic and mammary carcinomas. It is not expressed in lymphblastoid cells.

Spécificité

Recognizes Integrin beta5. Specificity determined by immunoprecipitation from detergent extract of 35S methionine labeled cells. No cross-reactivity to beta1 or beta3.

Immunogène

Epitope: C-terminus/cytosolic
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human integrin beta 5 subunit (KTFNKFNKSYNGTVD). Note : a lysine (K) residue was added to the N-terminal of the peptide to facilitate coupling to the carrier protein.

Application

Detect Integrin β5 using this Anti-Integrin β5 Antibody, C-terminus/cytosolic validated for use in ELISA, IF, IH(P), IP, RIA & WB.
ELISA/RIA

Immunoprecipitation: 5μL of antibody is sufficient to precipitate alphaVbeta5 from

2.5x10E6 cells.

Western blot: non-reduced conditions; do not use milk in the dilution buffer.

Immunofluorescence: 1:100.

Immunohistochemistry (Acetone Fixed) 1:40-1:80

Immunohistochemistry (Paraffin Embedded) 1:200 (ABC)

Optimal working dilutions must be determined by end user.

Description de la cible

88 kDa

Forme physique

UnPurified whole rabbit Serum containing 0.05% sodium azide as a preservative.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Syndecan-1 regulates alphavbeta5 integrin activity in B82L fibroblasts.
McQuade, KJ; Beauvais, DM; Burbach, BJ; Rapraeger, AC
Journal of Cell Science null
Integrins control dendritic spine plasticity in hippocampal neurons through NMDA receptor and Ca2+/calmodulin-dependent protein kinase II-mediated actin reorganization.
Shi, Y; Ethell, IM
The Journal of Neuroscience null
Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
Yan, YX; Gong, YW; Guo, Y; Lv, Q; Guo, C; Zhuang, Y; Zhang, Y; Li, R; Zhang, XZ
Testing null
Molecular consequences of silencing mutant K-ras in pancreatic cancer cells: justification for K-ras-directed therapy.
Fleming, JB; Shen, GL; Holloway, SE; Davis, M; Brekken, RA
Molecular Cancer Research null
J Watzlawik et al.
Glia, 58(15), 1782-1793 (2010-07-21)
Human remyelination promoting IgM mAbs target oligodendrocytes (OLs) and function in animal models of multiple sclerosis (MS). However, their mechanism of action is unknown. This study seeks to identify the cellular mechanism of action of a recombinant human IgM on
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