AB1727
Anti-Connexin 43 Antibody
Chemicon®, from rabbit
Synonyme(s) :
Gap Junction alpha-1 Protein (CxA-1)
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, bovine, rat
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GJA1(2697)
Description générale
Mouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin
a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
Spécificité
Mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunogène
KLH-conjugated synthetic peptide
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.
Application
ELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43.
Forme physique
Affinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide.
Stockage et stabilité
Store at 2-8°C for 1 year from date of receipt.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Brant E Isakson et al.
Circulation research, 97(1), 44-51 (2005-06-18)
Heterocellular communication between vascular smooth muscle cells (VSMC) and endothelial cells (EC) at the myoendothelial junction (MEJ) is a critical part of control of the arteriolar wall. We have developed an in vitro model of the MEJ composed of primary
J E Rash et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(6), 1983-2000 (2001-03-14)
The transmembrane connexin proteins of gap junctions link extracellularly to form channels for cell-to-cell exchange of ions and small molecules. Two primary hypotheses of gap junction coupling in the CNS are the following: (1) generalized coupling occurs between neurons and
Khai Le Quang et al.
Circulation. Arrhythmia and electrophysiology, 8(4), 921-932 (2015-06-14)
Integrin-linked kinase (ILK), a serine/threonine protein kinase, has roles in cell signaling and molecular scaffolding. ILK mutation/deletion causes cardiomyopathic phenotypes, but the functional and electrophysiological features have not been characterized. This study investigated the structural, functional, ion channel, and electrophysiological
Jean-Baptiste Guichard et al.
Cardiovascular research, 117(2), 462-471 (2020-01-25)
No studies have assessed the specific contributions of atrial fibrillation (AF)-related atrial vs. associated ventricular arrhythmia to remodelling. This study assessed the roles of atrial arrhythmia vs. high ventricular rate in AF-associated remodelling. Four primary dog-groups (12/group) were subjected to 3-week
Elisa Ferraro et al.
Scientific reports, 10(1), 15284-15284 (2020-09-19)
Acute myocardial ischaemia and reperfusion (I-R) are major causes of ventricular arrhythmias in patients with a history of coronary artery disease. Ursodeoxycholic acid (UDCA) has previously been shown to be antiarrhythmic in fetal hearts. This study was performed to investigate
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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