553514-M

RAD51 Inhibitor II, RI-1

The RAD51 Inhibitor, RI-1 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonyme(s) : RAD51 Inhibitor II, RI-1, 3-chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione
• Formule empirique (notation de Hill): C₁₄H₁₁Cl₃N₂O₃
• Poids moléculaire : 361.61
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Pureté: ≥99% (HPLC)
• Forme: solid
• Puissance: 5-30 μM IC₅₀
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: bright yellow
• Solubilité: DMSO: 50 mg/mL
• Température de stockage: 2-8°C

Description

Description générale

A cell-permeable 1H-Pyrrole-2,3-dione derivative that covalently binds to RAD51 at Cys319 and irreversibly destabilizes a protein-protein interface that is essential for filament formation and recombinase activity. Stimulates single strand annealing at a 5-30 µM concentration. Inhibits subnuclear RAD51 foci formation following DNA damage without affecting replication protein A. Also, effectively sensitizes cancer cells to mitomycin C (~25 µM) and generate synergistic cell kill in RAD51C mutant cells and with lesser effect on RAD51 complemented cells. Satisfies all four Lipinski rules for predicting drug likeness.

Actions biochimiques/physiologiques

Primary Target
RAD51

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Budke, B., et al. 2012. Nucleic Acids Res.40, 7347.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable