345872

Glial Derived Neurotrophic Factor, Human, Recombinant, E. coli

• Synonyme(s) : rhGDNF
• Code UNSPSC : 12352202

Propriétés

• Essai: ≥97% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Solubilité: aqueous buffer: soluble (most); water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Recombinant, human glial derived neutotrophic factor expressed in E. coli. A heterogeneously glycosylated growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. Activates receptor tyrosine kinase Ret and promotes kidney morphogenesis. Also an activator of MAP kinase. Its biological activity is destroyed by reduction of disulfide bonds.

Recombinant, human glial derived neutotrophic factor expressed in E. coli. Growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. A heterogeneously glycosylated basic protein. Its biological activity is destroyed by the reduction of disulfide bonds.

Actions biochimiques/physiologiques

fully biologically active when compared to native GDNF by dose-dependent ³H-dopamine uptake assay

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

The recommended stock solution is 1 µg/100µl. For more dilute samples, add a stabilizing protein such as 0.1% BSA.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Vega, Q.C., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10657.
Worby, C.A., et al. 1996. J. Biol. Chem. 271, 23619.
Lin, L.F., et al. 1994. J. Neurochem. 63, 758.
Lev-Fen, H., et al. 1993. Science260, 1130.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany