218725

Caspase-5, Human, Recombinant, E. coli

Caspase-5, Human, Recombinant, is expressed in E. coli. It is an inflammatory caspase.

• Synonyme(s) : ICErel-III, TY
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Essai: ≥80% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥100,000 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

M.W. 10,000 and 22,000 (heterodimer).

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

Recombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.

Forme physique

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

After initial defrost, aliquot and freeze at -70°C.

Autres remarques

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2