16-105
Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate
clone 4G10®, Upstate®, from mouse
Synonyme(s) :
Anti-phosphotyrosine antibody
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.56
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
4G10®, monoclonal
Réactivité de l'espèce (prédite par homologie)
all
Fabricant/nom de marque
Upstate®
Technique(s)
ELISA: suitable
western blot: suitable
Isotype
IgG2bκ
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pTyr)
Informations sur le gène
human ... PID1(55022)
Description générale
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Spécificité
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogène
Phosphotyramine-KLH.
Application
Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in ELISA & WB.
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Qualité
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Description de la cible
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Forme physique
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% Kathon® . Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Protein G-Sepharose Chromatography
Stockage et stabilité
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
Remarque sur l'analyse
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Informations légales
4G10 is a registered trademark of Upstate Group, Inc.
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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