14-602-M
PI3 Kinase (p110α/p85α) Protein, active, 20 µg
Active complex of N-terminal His6-tagged recombinant full-length human p110α & untagged, recombinant, full length, human p85α. For use in Kinase Assays.
Synonyme(s) :
Active PI3 Kinase Protein, PI3 Kinase p110α/p85α
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.26
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in baculovirus infected Sf21 cells
Poids mol.
Mw 125.3 kDa (p110α)
Mw 83.7 kDa (p85α)
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Numéro d'accès NCBI
Numéro d'accès UniProt
Informations sur le gène
human ... PIK3CA(5290)
Description générale
Product Source: Coexpressed by baculovirus in Sf21 insect cells
Research area: Cell signaling
Protein Target: PI3Kα/p85α Target Sub-Family: PI3K/PI4K
Complex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α
Protein Target: PI3Kα/p85α Target Sub-Family: PI3K/PI4K
Complex of N-terminal His6-tagged recombinant full-length human p110α and untagged, recombinant, full length, human p85α
Application
PI3 Kinase (p110α/p85α) Protein has been used in in vitro kinase assay.
Actions biochimiques/physiologiques
Phosphoinositide 3-kinases (PI3Ks) are enzymes that play a crucial role in cell signaling. They are responsible for activating signaling pathways that regulate important cellular processes such as cell survival, proliferation, protein synthesis, cell metabolism, cytoskeletal rearrangements, and vesicle trafficking. PI3Ks exhibits both lipid kinase activity (phosphorylation of inositol ring in phosphatidylinositol (PtdIns) lipids) and protein kinase activity (including autophosphorylation).
Conditionnement
Also available in 250μg size as catalog # 14-602M - Call for pricing and availability.
Qualité
routinely evaluated by PIProfiler
Forme physique
Ni2+/NTA-agarose
Stockage et stabilité
6 months at -70°C
Autres remarques
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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PKCalpha reduces the lipid kinase activity of the p110alpha/p85alpha PI3K through the phosphorylation of the catalytic subunit
Sipeki S, et al.
Biochemical and Biophysical Research Communications, 339(1), 122-125 (2006)
Kinetic and structural analyses reveal residues in phosphoinositide 3-kinase ? that are critical for catalysis and substrate recognition
Maheshwari S, et al.
The Journal of biological chemistry, 292(33), 13541-13550 (2017)
Christina M Buchanan et al.
PloS one, 8(8), e71337-e71337 (2013-08-13)
In addition to lipid kinase activity, the class-I PI 3-kinases also function as protein kinases targeting regulatory autophosphorylation sites and exogenous substrates. The latter include a recently identified regulatory phosphorylation of the GM-CSF/IL-3 βc receptor contributing to survival of acute
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