07-1519

Anti-phospho Grb10 (Ser503) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): primate (based on 100% sequence homology), porcine (based on 100% sequence homology), human (immunogen homology)
• Technique(s): inhibition assay: suitable (peptide); western blot: suitable
• Numéro d'accès NCBI: NP_005302
• Numéro d'accès UniProt: Q13322
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer503)
• Informations sur le gène: human ... GRB10(2887) ; mouse ... Grb10(14783) ; pig ... Grb10(100188977)

Description

Description générale

Grb10 (insulin receptor-binding protein Grb-IR) is a ubiquitous adapter protein which is expressed in both fetal and adult tissues. It is a multidomain protein that interacts with a wide array of cellular targets; the PH domain allows interaction with several subtypes of phosphoinositides; the Ras-associating domain binds a wide variety of Ras GTPases; and the SH domain facilitates binding to membrane-bound tyrosine kinase receptors which link Grb10 to different signaling pathways. Most notably, Grb10 associates with the insulin receptor at phosphotyrosine residues via its C-terminal BPS (between PH and SH) domain and inhibits the enzymatic activity of the insulin receptor. Grb10 also acts downstream of the insulin receptor pathway to inhibit activation of AKT-1. In addition, Grb10 suppresses Wnt signalling by binding to the low-density lipoprotein receptor. In fetal tissues, Grb10 may inhibit growth and placental size.

Spécificité

Other homologies: Rat (91% sequence homology).

This antibody recognizes Grb10 phosphorylated at (Ser503).

Immunogène

Epitope: Phosphorylated (Ser503)

KLH-conjugated linear peptide corresponding to human GRB10 phosphorylated at (Ser503).

Application

Peptide Inhibition Assay Analysis: 1 µg/mL from a representative lot detected phospho-Grb10 (Ser503) on 10 µg of embryonic fibroblast cell lysate + non-phospho-Grb10 (Ser503) peptide. However, the antibody was inhibited when incubated with a phospho-Grb10 (Ser503) peptide.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-phospho Grb10 (Ser503) Antibody is validated for use in Peptide Inhibition Assay, Western Blotting for the detection of phospho Grb10 (Ser503).

Qualité

Evaluated by Western Blot in mouse embryonic fibroblast cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-Grb10 (Ser503) on 10 µg of mouse embryonic fibroblast cell lysate.

Description de la cible

~75 kDa observed. An uncharacterized band appears at ~16 kDa in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse embryonic fibroblast cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable