06-1074

Anti-Interleukin 21 (IL-21) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Za11, IL-21, IL21, Interleukin-21
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_068575
• Numéro d'accès UniProt: Q9HBE4
• Conditions d'expédition: wet ice
• Informations sur le gène: human ... IL21(59067)

Description

Description générale

Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin′s lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin′s lymphoma including clusters of other immune cells gathered around HL cells in cultures.

Spécificité

Cow, canine, equine, and porcine (93% sequence homology). Rat (86% sequence homology).

This antibody recognizes the C-terminus of IL-21.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-21.

Application

Anti-Interleukin 21 (IL-21) Antibody is an antibody against Interleukin 21 (IL-21) for use in FC, IH(P) & WB.

Flow Cytometry Analysis: 5 µg from a previous lot detected IL-21 in human PBMC′s.

Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-21 in mouse spleen tissue.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Qualité

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-21 on 10 µg of K562 cell lysate.

Description de la cible

A molecular weight of 18 kDa was observed; however, UniProtKB/Swiss-Prot entry Q9HBE4 (IL21_HUMAN) describes 2 isoforms produced by alternative splicing: isoform 1 at 18 kDa, isoform 2 at 17 kDa

Forme physique

Affinity purified

Purified rabbit polyclonal in PBS with 0.06% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
K562 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10-13 - German Storage Class 10 to 13