810601C

16:0-5 Doxyl PC

Avanti Research^™ - A Croda Brand 810601C

• Synonyme(s) : 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine
• Formule empirique (notation de Hill) : C₄₆H₉₀N₂O₁₀P
• Numéro CAS: 188004-24-2
• Poids moléculaire : 862.19
• Numéro MDL: MFCD02259246
• Code UNSPSC : 51321705
• Nomenclature NACRES : NA.25

Propriétés

• Essai: >99% (TLC)
• Forme: liquid
• Conditionnement: pkg of 1 × 1 mL (810601C-1mg)
• Fabricant/nom de marque: Avanti Research^™ - A Croda Brand 810601C
• Concentration: 1 mg/mL (810601C-1mg)
• Type de lipide: ESR probes; phospholipids
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins[1] and as fluorescent quenchers in lipid bilayer structural studies.[2]

Phosphatidylcholine (PC), a strong bilayer-forming lipid is the most common phospholipid in mammalian membranes.[3] It is also an important component of the mucosal layer of the colon.[4] The 5^th carbon of the sn-2 stearic acid chain of 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine analog has a Doxyl PC, a spin probe[5] attached to it covalently.[6]

Application

16:0-5 Doxyl PC may be used:

• as a component in virus-like large unilamellar vesicles (VL LUVs) to quench 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence emission[7]
• in the preparation of multi-lamellar vesicles (MLVs) as a site-specific quencher to perform fluorescence quenching studies[8]
• in the preparation of spin-labelled multi-lamellar vesicles (MLVs)[9]

Actions biochimiques/physiologiques

Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.[4] It is used to treat fat embolism. Phosphatidylcholine lowers the levels of cholesterol and triglycerides.[10]

Conditionnement

5 mL Clear Glass Sealed Ampule (810601C-1mg)

Notes préparatoires

To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies.[11] For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM.[2], Supplemental information

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• Conseils de prudence: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Classification des risques: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Organes cibles: Central nervous system, Liver,Kidney
• Classe de danger pour l'eau (WGK): WGK 3