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330002S

Avanti

E06 mAb-biotinylated

E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA

Synonyme(s) :

E06 mAb-biotinylated, T15 antibody

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.45

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Essai

>95%

Forme

solution

Conditionnement

pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))

Fabricant/nom de marque

Avanti Research™ - A Croda Brand 330002S

Concentration

0.2 mg/mL (330002S-25ug)

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Application

E06 mAb-biotinylated has been used in immunodetection. It may be used in immunohistochemistry, western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).

Actions biochimiques/physiologiques

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL), oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys, mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.

Conditionnement

0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.
Palinski W, et al.
The Journal of Clinical Investigation, 98(3), 800-814 (1996)
Increased plasma oxidized phospholipid: apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression
Tsimikas S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), 175-181 (2007)
Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells
Coliva G, et al.
Redox Biology, 23, 101123-101123 (2019)

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