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W0876

Sigma-Aldrich

White′s Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

White′s Basal Salt Mixture is widely used in plant tissue culture.

Application

White′s Basal Salt Mixture has been used:
  • as a medium for the precultured spores of Prothalli of Adiantum capillus for the generation of protonemata
  • to promote germination and cotton cotyledon unfolding
  • as a solidified medium component for culturing A. capillus and as a component of selection medium for gametophyte selection after particle bombardment

Formula variant

With the macro- and micronutrients as described by White (1968).

Media Formulation

Signal Word

Danger

Hazard Statements

H272,H318,H373,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Ox. Sol. 3 - STOT RE 2 Inhalation

Target Organs

Brain

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hiroko Kawai-Toyooka et al.
Plant & cell physiology, 45(11), 1648-1657 (2004-12-03)
RNA interference (RNAi) has become a powerful tool for determining gene function and is used in a wide variety of organisms. Since it is necessary to generate double-stranded RNA (dsRNA) as an inducer for RNAi, preparation of RNAi-inducing constructs is
White's standard nutrient solution
SINGH M and Krikorian AD
Annals of Botany, 47, 133-139 (1981)
K Rajasekaran
Methods in molecular biology (Clifton, N.J.), 1902, 35-45 (2018-12-14)
Biolistic transformation of cotton (Gossypium hirsutum L.) meristems, isolated from mature seed, is detailed in this report. A commercially available, helium-driven biolistic device (Bio-Rad PDS1000/He ) was used to bombard gold particles coated with a marker gene (uidA or "GUS")
S J Sata et al.
Methods in cell science : an official journal of the Society for In Vitro Biology, 22(4), 299-304 (2001-09-11)
An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was
D Parfitt et al.
Journal of experimental botany, 55(406), 2169-2177 (2004-09-14)
If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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