T9533

Transforming Growth Factor-α from rat

powder, synthetic, γ-irradiated, suitable for cell culture

• Synonym(s): TGF-α
• Empirical Formula (Hill Notation): C₂₄₄H₃₆₇N₇₁O₇₁S₆
• CAS Number: 89899-53-6
• Molecular Weight: 5623.35
• MDL number: MFCD00082077
• UNSPSC Code: 12352200

Properties

• biological source: synthetic
• sterility: γ-irradiated
• Assay: ~90% (HPLC)
• form: powder
• potency: 0.02-2.0 ng/mL EC₅₀ (using Balb/MK 1)
• mol wt: 5.5 kDa
• composition: Peptide content, ~80%
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P01134
• storage temp.: −20°C
• Gene Information: rat ... Tgfa(24827) , Tgfa(24827)

Description

Amino Acid Sequence

Val-Val-Ser-His-Phe-Asn-Lys-Cys-Pro-Asp-Ser-His-Thr-Gln-Tyr-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Glu-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Val-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide Bridges: 8-21; 16-32; 34-43]

Biochem/physiol Actions

Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves