T8578

Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal

~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture

• Synonym(s): Anti-β-Tubulin Class III, Anti-Neuron-specifc class III β-tubulin, Anti-TUBB4, Anti-Tubb3, Anti-Tubulin β-4, Anti-Tubulin β-III, Anti-Tubulin, β−3
• MDL number: MFCD01323870
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 2G10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human, mouse, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using PC12 cell extract
• isotype: IgG2a
• UniProt accession no.: Q13509
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB3(10381) ; mouse ... Tubb3(22152) ; rat ... Tubb3(246118)

Description

General description

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.

Application

Monoclonal Anti-β-Tubulin III (neuronal) antibody produced in mouse has been used in following studies:

• Immunofluorescence.
• Immunocytochemistry.

Biochem/physiol Actions

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves