Tyrode′s Salts solution is designed to work with mammalian cells. This balanced salt solution can be used for diluting protein components of media of natural origin. Balanced Salt solution plays a role in providing a buffer system to maintain the medium in optimum pH range (7.2-7.6), irrigating and diluting while maintaining the intra- and extracellular osmotic balance. It facilitates the maintenance of normal cell metabolism by giving water and bulk inorganic ions. Balanced Salt solution provides the energy source for cell metabolism by supplying nutrients.
Application
Tyrode′s Salts has been used:
to incubate sperms collected from cauda epididymis of rat, mouse, guinea pig, golden hamster and rabbit [1][2]
for the transportation of lower uterine segment (LUS) strips from women[3]
for the transportation of Cor.4U human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes[4]
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During mouse embryonic development, vasculogenesis initially occurs in the yolk sac, preceding neurulation. Our previous studies have demonstrated that maternal diabetes induces embryonic vasculopathy at early embryonic developmental stage by suppressing the expression of vascular growth factors including BMP4 (bone
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Calcium-based screening of hiPS-CMs is a useful preclinical safety evaluation platform with the ability to generate robust signals that facilitates high-throughput screening and data analysis. However, due to the potential inherent toxicities, it is important to understand potential effects of
Failure of neural tube closure results in severe birth defects and can be induced by high glucose levels resulting from maternal diabetes. MARCKS is required for neural tube closure, but the regulation and of its biological activity and function have
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Sperm morphology mainly refers to the shape of the head, the length of the flagellar segments, including the midpiece, principal piece and end piece, and the size of the accessory structures, including axonemes, outer dense fibers (ODFs), mitochondrial sheath (MS)
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