SRP0429
PDE4A10 active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥75% (SDS-PAGE)
Synonym(s):
DPDE2, PDE46, phosphodiesterase 4A, cAMP-specific
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥75% (SDS-PAGE)
form
aqueous solution
specific activity
≥75% (compared to standard)
mol wt
117 kDa
packaging
pkg of 10 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE4A(5141)
General description
Human PDE4A10, also known as PDE4A protein isoform 6, transcript variant 3 (GenBank Accession No. NM_001111309) amino acids 2-825 (end) with N-terminal GST-tag, MW= 117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cAMP to 5?-AMP per min at 37°C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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I McPhee et al.
Cellular signalling, 13(12), 911-918 (2001-12-01)
In situ hybridisation methods were used to map the distribution of the novel long PDE4A10 isoform in the brain. PDE4A10 distribution was compared to that of the long PDE4A5 isoform and the short PDE4A1 isoform using probes specific for unique
Angela McCahill et al.
Cellular signalling, 20(11), 2071-2083 (2008-08-30)
Transcripts for the PDE4A10 cyclic AMP phosphodiesterase isoform are present in a wide variety of rat tissues including the heart. Sequence comparisons between the putative human and mouse promoters revealed a number of conserved regions including both an Sp1 and
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