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SAB4200380

Sigma-Aldrich

Anti-LAYN antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-LAYN antibody produced in rabbit, Anti-Layilin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using human A549 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LAYN.

UniProt accession no.

Q6UX15

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LAYN(143903)

General description

Layilin (LAYN) is a cell surface hyaluronan receptor, originally identified as a binding partner of talin located in membrane ruffles of spreading cells. LAYN is widely expressed in different cell lines and tissues. This gene is mapped to human chromosome 11q.

Immunogen

synthetic peptide corresponding to an internal region of human LAYN, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-LAYN antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Layilin (LAYN) binds to hyaluronan, a ubiquitous extracellular matrix component involved in the invasion of a variety of tumour cells, through its extracellular domain and to cytoskeleton-membrane linker proteins including Merlin and Radixin, through its cytoplasmic domain, implicating LAYN in cell adhesion and motility. LAYN is overexpressed in the human lung carcinoma A549. LAYN down-regulation by RNA interference, inhibits invasion and lymphatic metastasis of A549 cells.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells
Chen Z, et al.
Biotechnology and Applied Biochemistry, 50(Pt), 2-2 (2008)
Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin
Bono, et al.
Experimental Cell Research, 308(1) (2005)
Stefan Deneberg et al.
Epigenetics, 9(6), 910-917 (2014-04-02)
A commonly deleted region in chronic lymphocytic leukemia (CLL) is the 11q22-23 region, which encompasses the ATM gene. Evidence suggests that tumor suppressor genes other than ATM are likely to be involved in CLL with del(11q). A microRNA (miR) cluster

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