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S5150

Sigma-Aldrich

Sodium chloride solution

5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

Synonym(s):

Halite, NaCl, sodium chloride

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1 L
€91.80
4 L
€311.00

€91.80

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1 L
€91.80
4 L
€311.00

About This Item

Linear Formula:
NaCl
CAS Number:
7647-14-5
Molecular Weight:
58.44
Beilstein:
3534976
EC Number:
231-598-3
MDL number:
MFCD00003477
UNSPSC Code:
12352302
PubChem Substance ID:
24899641
NACRES:
NA.25
grade:
for molecular biology
form:
liquid

€91.80

Please contact Customer Service for Availability
Request a Bulk Order

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H2O

technique(s)

ChIP: suitable
DNA extraction: suitable
cell culture | mammalian: suitable

impurities

DNAse, none detected
 Endonuclease, none detected
 Exonuclease, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected
<1.0 EU/mL Endotoxin level
<16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm3

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm
heavy metals (as Pb): ≤5 ppm

suitability

suitable for Western blot
suitable for molecular biology

application(s)

agriculture
agriculture

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA.[1] It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.[2]

Preparation Note

Prepared in 18 megohm water.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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S6150

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71376

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000332879
0000332879
0000411532
0000411532
0000388971

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Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice
Vassileva G, et al.
The Journal of Endocrinology, 205(3), 225-232 (2010)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
Francis Kim et al.
JAMA, 311(1), 45-52 (2013-11-19)
Hospital cooling improves outcome after cardiac arrest, but prehospital cooling immediately after return of spontaneous circulation may result in better outcomes. To determine whether prehospital cooling improves outcomes after resuscitation from cardiac arrest in patients with ventricular fibrillation (VF) and
Alexander W Wyatt et al.
Genome biology, 15(8), 426-426 (2014-08-27)
Genomic analyses of hundreds of prostate tumors have defined a diverse landscape of mutations and genome rearrangements, but the transcriptomic effect of this complexity is less well understood, particularly at the individual tumor level. We selected a cohort of 25
I Dzneladze et al.
Leukemia, 29(7), 1485-1495 (2015-03-05)
In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
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Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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